That's frustrating! I don't know if my experience will help, but I've had issues like this in the past using UseMyBank - and basically I had to deal with three separate entities, the casino, the intermediary (in my case UseMyBank), and the bank.
Doesn't Ladbroke's have live chat? Get online with them and see what they say. If they're saying that the funds didn't go through, then contact the intermediary - it's possible that they took the funds from your bank but they're stuck in limbo somewhere in pending status until clearing. If the intermediary says that the funds never came from your bank, then contact your bank. If the funds were deducted from your bank, the bank with have a transaction ID - you send that ID to the intermediary and wait for them to find it. At the same time, your bank will look into the transaction but it usually takes them a lot longer.
My last issue with UseMyBank it took them a couple of days to find the funds once they had the transaction ID from my bank. Then about a month after that I got a call from UseMyBank saying that they'd just received notice from my bank that they owed me a refund - by that time the money was long gone. 
Good luck!
"I feel sullied and unusual" - Captain Jack Sparrow
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